Application and quantification of mechanical load to a 3D bone-tissue analogue within a microfluidic device – bone-on-a-chip
In this project we develop a microfluidic bioreactor that is able to apply and quantify mechanical load and to control oxygen levels. In addition, control software allowing automated operation of up to four units in parallel will be employed. The bioreactor will be combined with different bone organoids that resemble intramembranous and endochondral ossification, thereby modelling bone development and regeneration under physiological conditions. Within this project, primary bone cells will be used to tissue engineer the respective organoids.