EUROoC comprises a collection of innovative research projects addressing the development of advanced OoC systems with higher physiological significance going beyond current in vitro testing. The EUROoC project will create advanced OoCs, which closely recapitulate properties of the respective organ tissues in vivo regarding cell types, microenvironment, organ-specific tissue structure and function as well as concepts for the interconnection of individual OoCs.
The various OoC models to be developed comprise heart-on-a-chip, bone-on-a-chip, retina-on-a-chip, lung-on-a-chip, adipose- on-a-chip, guton-a-chip to liver-on-a-chip. The OoC systems will be able to monitor and analyse tissue functionality and response in situ by integrating various novel sensing elements. The OoCs developed will be tested with the regulatory partners through in vitro-in vivo correlations.
Organ-on-Chip and the 3R: A virtual lab tour
Our 15 PhD students give you a virtual lab tour in 11 laboratories involved in the EUROoC International Training Network, where they are working on finding alternatives to reduce animal testing. You are guided through the processes of building and setting-up an organ-on-chip experiment for in vitro modeling and drug screening. 4 PhD students will highlight their chips and applications.
Parallelisable microfluidic Heart-on-a-chip systems with integrated sensing capability to monitor maturation and functionality of cardiac microtissues
Application and quantification of mechanical load to a 3D bone-tissue analogue within a microfluidic device – bone-on-a-chip
Development of a microphysiological system that integrates and differentiates tissue-specific ECs
Development of a microfludic-based OoC model as a device for modelling cellular therapy approaches
Gut-on-a-chip modelling microbiome dysbiosis
Retina-on-Chip with Local Oxygen Sensing
Development of an acute lung infection model
Microfluidic methods for bead-based assays on-chip
Development of defined matrices for next-generation OoC systems
Microvasculature-on-a-chip modelling degenerative vascular disease
Establishment of a liver zonation and infection model
Integration of on-chip TEER measurement and of iPSC-derived epithelial cells in the HuMiX gut/microbiome model
Human immunocompetent WAT-Liver-on-a-chip system with integrated sensors
Optical sensor systems for multi-parametric monitoring and control of OoCs
Optical sensor concepts for monitoring metabolic activity
M. Lucchetti, M. Kaminska, A. Kehinde Oluwasegun, Alexander S. Mosig, P. Wilmes.
Emulating the gut–liver axis: Dissecting the microbiome’s effecton drug metabolism using multiorgan-on-chip models
Publication: Current Opinion in Endocrine and Metabolic Research, 2021, 18:94–101.
M. R. Schneider, M. Oelgeschlaeger, T. Burgdorf, P. van Meer, P. Theunissen, A. S. Kienhuis, A. H. Piersma, R. J. Vandebriel.
Applicability of organ-on-chip systems in toxicology and pharmacology
Publication: Critical Reviews in Toxicology, 2021.
S. Fuchs, S. Johansson, A. Tjell, G. Werr, T. Mayr, M. Tenje
In-Line Analysis of Organ-on-Chip Systems with Sensors: Integration, Fabrication, Challenges, and Potential
Publication: ACS Biomaterials Science & Engineering, 2021, 7, 7, 2926–2948.
U. Arslan, V.V. Orlova, C.L. Mummery.
Perspectives for Future Use of Cardiac Microtissues from Human Pluripotent Stem Cells
Publication: ACS Biomaterials Science & Engineering, 2021.
T. Shroff, K. Aina, C. Maass, M. Cipriano, J. Lambrecht, F. Tacke, A. Mosig, P. Loskill.
Studying metabolism with multi-organ chips: new tools for disease modelling, pharmacokinetics and pharmacodynamics
Publication: Open Biology, 2022.
U. Arslan, A. Moruzzi, J. Nowacka, C.L. Mummery, D. Eckardt, P. Loskill, V.V. Orlova
Microphysiological Stem Cell Models of the Human Heart
Publication: Materials Today Bio, 2022, 14.
O. Schneider, A. Moruzzi, S. Fuchs, A. Grobel, H.S. Schulze, T. Mayr, P. Loskill
Fusing spheroids to aligned μ-tissues in a heart-on-chip featuring oxygen sensing and electrical pacing capabilities
Publication: Material Today Bio, 2022, 15.
F. Cantoni, G. Werr, L. Barbe, A. Porras, M. Tenje
A microfluidic chip carrier including temperature control and perfusion system for long-term cell imaging
Publication: HardwareX 10 (2021): e0024.
S. Fuchs, R.W.J. van Helden, M. Wiendels, M.N.S. de Graaf, V.V. Orlova, C.L. Mummery, B.J. van Meer, T. Mayr
On-chip analysis of glycolysis and mitochondrial respiration in human induced pluripotent stem cells
Publication: Materials Today Bio (2022): 17.